Absolute Quantification


The Problem:

Obtaining absolute PCR quantification currently requires the laborious preparation of standards and acquiring a standard curve, thereby wasting reagents and using valuable plate real estate. In addition, the traditional analysis method for qPCR determines the "cycle threshold", Ct or Cq, which varies for different assays, different machines, and varies from plate to plate, thereby making the Ct value hard to interpret.

 

The Solution:

DNA Software has made a breakthrough in understanding the mechanism of PCR amplification. Our new product, qPCR CopyCount™, allows for any qPCR curve to be analyzed to directly determine the absolute number of copies of DNA at cycle zero. The DNA copy count is the quantity that every biologist wants and the results provided by qPCR CopyCount have unprecedented relative and absolute accuracy.  Click here to watch a video seminar on Copy Count.

 

How secure is CopyCount Cloud Edition?

Absolute PCR Quantification with existing qPCR instruments

Utilizing your current lab equipment and methods, and providing you higher sample throughput than Digital PCR, qPCR CopyCount™ provides absolute DNA copy number quantification with outstanding accuracy, within 5% error. Rather than the need for expensive new equipment, qPCR CopyCount™ makes every qPCR reaction an absolute reaction.

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Lower Cost Per Well

qPCR CopyCount™ requires no quantification standards and provides users more sample wells rather than control wells. In other words, “more real estate for your plate”.

DNA Copy Number from qPCR Data

Obtain absolute quantification and DNA Copy Number of your qPCR data within 5% errors.

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Our Latest

Thursday, 06 November 2014 22:15

DNA Software introduced the full commercial release of ThermoBLAST Cloud Edition (TB-CE). TB-CE provides a new standard for evaluating the target specificity of oligonucleotides.  Click here to learn more.  

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“It [Visual OMP™] is very powerful software when used for multiplexing design...” Chris Novak, Ambion
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“I have been using DNA [Software™] for a long time, at least 8 years. I want to have the best tools available and that is why we use it.” Dr. Nancy Schoenbrunner, Roche Molecular Systems
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“In my experience, DNA Software™ saved me 75% of my oligo costs.” Helen Minnis, Wave 80
“DNA [Software™] has passed my tests. I’ve recommended it [Visual OMP™]. It performs extremely well.” Dr. Ned Sekinger, Luminex Corporation
“Learning to use the program [Visual OMP™] is time well spent, and the support staff at DNA Software is always ready and able to help” Dr. Sue J. Kohlhepp, Providence Portland Medical Center.